2018 AANS Annual Scientific Meeting-511. CRISPR Improved: Inducible CRISPR-Interference Achieves Specific and Reversible PIKE-A Silencing in Glioblastoma

511. CRISPR Improved: Inducible CRISPR-Interference Achieves Specific and Reversible PIKE-A Silencing in Glioblastoma

Video Transcription

Video Summary

In this video, Arman Jahangiri presents his talk on CRISPR-improved inducible CRISPR interference, specifically looking at the silencing of the PIK-A gene in glioblastoma. He explains that traditional methods of gene knockout and knockdown can be nonspecific and irreversible, but CRISPR-I provides a tunable system for downregulating individual genes. In their study, they used CRISPR-I to repress PIK-A gene expression in glioblastoma cells and achieved a knockdown of over 99%. They also observed a significant reduction in invasion and proliferation rates in the cells with PIK-A knockdown. They suggest that CRISPR-I can be a valuable tool for studying and targeting essential genes in cancer cells.

Asset Caption

Arman Jahangiri

Keywords

CRISPR-improved inducible CRISPR interference

PIK-A gene

glioblastoma

gene knockout

gene knockdown