2018 AANS Annual Scientific Meeting-642. Unique Nascent RNA Sequences that Distinguish Normal Brain and Malignant Gliomas

642. Unique Nascent RNA Sequences that Distinguish Normal Brain and Malignant Gliomas

Video Transcription

Video Summary

Dr. Lawrence Chin discusses the unique nascent RNA sequences that differentiate normal brain cells from clinical neurons. He explains that the human genome is mostly non-coding, with only 2% expressing gene products. Regulatory sequences, which are separate from the gene itself, play a crucial role in gene expression. Dr. Chin introduces ProSeq and CroSeq, techniques developed at Cornell University that use RNA polymerase II to transcribe and sequence nascent RNA, providing a comprehensive description of the DNA. This technique allows for complete evaluation of the genome and can identify regulatory sequences. The CroSeq technique has been tested on brain tumor samples from as far back as 1988, revealing differential gene expression and similarity to normal brain tissue. The technique also identifies specific regulatory elements related to stem cell properties, making it a valuable tool in understanding tumor genome regulation. Dr. Chin expresses gratitude to Dr. Danko and the team at Cornell for developing the CroSeq technique.<br /><br />Note: The summary has been drawn from the transcript provided and does not include any visuals or audio content from the video.

Asset Caption

Lawrence S. Chin, MD, FAANS

Keywords

nascent RNA sequences

gene expression

CroSeq technique

regulatory sequences

tumor genome regulation